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tev protease site  (Addgene inc)


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    Structured Review

    Addgene inc tev protease site
    Tev Protease Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloning+site/pET+His6+LIC+cloning+vector+(2Bc-T)+(Plasmid+%2337236)/bio_rxiv__64898__2026__03__25__714350-229-14-18
    Average 93 stars, based on 19 article reviews
    tev protease site - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in Supplementary Table S1 and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
    Article Snippet: .. Materials Coding sequences of individual toll-like receptors (TLR) were amplified from complementary DNA of human and mouse macrophages using Phusion high-fidelity DNA polymerase (ThermoFisher) and cloned to the multiple-cloning site of the pAdTrack-CMV vector (a gift from Bert Vogelstein (Addgene plasmid number 16405; http://n2t.net/addgene:16405 ; RRID: Addgene_16405), which expresses green fluorescent protein (GFP) from an independent CMV promoter 57 . .. The coding sequence of the TLR chaperone protein UNC93B1 and co-receptor CD14 were similarly amplified from human macrophage cDNA and inserted to the pMSCV-neo and pMSCV-puro vectors, respectively (Clontech).

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Clone Assay:

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in Supplementary Table S1 and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
    Article Snippet: .. Materials Coding sequences of individual toll-like receptors (TLR) were amplified from complementary DNA of human and mouse macrophages using Phusion high-fidelity DNA polymerase (ThermoFisher) and cloned to the multiple-cloning site of the pAdTrack-CMV vector (a gift from Bert Vogelstein (Addgene plasmid number 16405; http://n2t.net/addgene:16405 ; RRID: Addgene_16405), which expresses green fluorescent protein (GFP) from an independent CMV promoter 57 . .. The coding sequence of the TLR chaperone protein UNC93B1 and co-receptor CD14 were similarly amplified from human macrophage cDNA and inserted to the pMSCV-neo and pMSCV-puro vectors, respectively (Clontech).

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: Investigating 3D microbial community dynamics of the rhizosphere using quantitative phase and fluorescence microscopy
    Article Snippet: .. We first cloned the desired inserts (i.e., promoters and mNeonGreen fluorophore) into the multiple cloning site of a pJM220 mini-Tn7 plasmid (Addgene, cat. ..

    Article Title: Engineering NIR-Sighted Bacteria
    Article Snippet: .. A version of Av NIRusk containing a multiple-cloning site rather than a fluorescent reporter was cloned by Gibson assembly and deposited at Addgene for distribution (accession number 235084). ..

    Article Title: Engineering NIR-sighted bacteria
    Article Snippet: .. A version of Av NIRusk containing a multiple-cloning site rather than a fluorescent reporter was cloned by Gibson assembly and deposited at Addgene for distribution (accession number 235084). ..

    Article Title: CRISPR-based screening of small RNA modulators of bile susceptibility in Bacteroides thetaiotaomicron
    Article Snippet: .. Briefly, we cloned ~750 bp of the regions upstream and downstream of the sRNA into the multiple cloning site of pSIE1 (a gift from Andrew Goodman, Addgene plasmid #136355; http://n2t.net/addgene:136355; RRID:Addgene_136355). ..

    Plasmid Preparation:

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in Supplementary Table S1 and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: LDL delivery of microbial small RNAs drives atherosclerosis through macrophage TLR8
    Article Snippet: .. Materials Coding sequences of individual toll-like receptors (TLR) were amplified from complementary DNA of human and mouse macrophages using Phusion high-fidelity DNA polymerase (ThermoFisher) and cloned to the multiple-cloning site of the pAdTrack-CMV vector (a gift from Bert Vogelstein (Addgene plasmid number 16405; http://n2t.net/addgene:16405 ; RRID: Addgene_16405), which expresses green fluorescent protein (GFP) from an independent CMV promoter 57 . .. The coding sequence of the TLR chaperone protein UNC93B1 and co-receptor CD14 were similarly amplified from human macrophage cDNA and inserted to the pMSCV-neo and pMSCV-puro vectors, respectively (Clontech).

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: Investigating 3D microbial community dynamics of the rhizosphere using quantitative phase and fluorescence microscopy
    Article Snippet: .. We first cloned the desired inserts (i.e., promoters and mNeonGreen fluorophore) into the multiple cloning site of a pJM220 mini-Tn7 plasmid (Addgene, cat. ..

    Article Title: JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.
    Article Snippet: c-Jun NH2-terminal protein kinase (JNK) and p38 are stress-activated mitogen-activated protein kinases (MAPK) that are phosphorylated by various stimuli.. It has been reported that the loss of desmoglein (DSG) 3, a desmosomal transmembrane core molecule, in keratinocytes impairs cell–cell adhesion accompanied by p38 MAPK activation.. To understand the biological role of DSG3 in desmosomes and its relationship with stress-activated MAPKs, we established DSG3 knockout keratinocytes (KO cells).

    Article Title: CRISPR-based screening of small RNA modulators of bile susceptibility in Bacteroides thetaiotaomicron
    Article Snippet: .. Briefly, we cloned ~750 bp of the regions upstream and downstream of the sRNA into the multiple cloning site of pSIE1 (a gift from Andrew Goodman, Addgene plasmid #136355; http://n2t.net/addgene:136355; RRID:Addgene_136355). ..

    Cloning:

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in Supplementary Table S1 and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli
    Article Snippet: .. Briefly, the wildtype promoter region of narUZYWV operon and argI was amplified using specific sets of primers listed in and after double digestion with KpnI and SalI the amplified fragments were cloned into pRU1097 vector with gfp as the reporter gene downstream of the multiple cloning site (gifted from Phillip Poole, Addgene plasmid# 14462). ..

    Article Title: Investigating 3D microbial community dynamics of the rhizosphere using quantitative phase and fluorescence microscopy
    Article Snippet: .. We first cloned the desired inserts (i.e., promoters and mNeonGreen fluorophore) into the multiple cloning site of a pJM220 mini-Tn7 plasmid (Addgene, cat. ..

    Article Title: JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.
    Article Snippet: c-Jun NH2-terminal protein kinase (JNK) and p38 are stress-activated mitogen-activated protein kinases (MAPK) that are phosphorylated by various stimuli.. It has been reported that the loss of desmoglein (DSG) 3, a desmosomal transmembrane core molecule, in keratinocytes impairs cell–cell adhesion accompanied by p38 MAPK activation.. To understand the biological role of DSG3 in desmosomes and its relationship with stress-activated MAPKs, we established DSG3 knockout keratinocytes (KO cells).

    Article Title: CRISPR-based screening of small RNA modulators of bile susceptibility in Bacteroides thetaiotaomicron
    Article Snippet: .. Briefly, we cloned ~750 bp of the regions upstream and downstream of the sRNA into the multiple cloning site of pSIE1 (a gift from Andrew Goodman, Addgene plasmid #136355; http://n2t.net/addgene:136355; RRID:Addgene_136355). ..

    Sequencing:

    Article Title: JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.
    Article Snippet: c-Jun NH2-terminal protein kinase (JNK) and p38 are stress-activated mitogen-activated protein kinases (MAPK) that are phosphorylated by various stimuli.. It has been reported that the loss of desmoglein (DSG) 3, a desmosomal transmembrane core molecule, in keratinocytes impairs cell–cell adhesion accompanied by p38 MAPK activation.. To understand the biological role of DSG3 in desmosomes and its relationship with stress-activated MAPKs, we established DSG3 knockout keratinocytes (KO cells).



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